Identification of Francisella tularensis in natural water samples by PCR
نویسندگان
چکیده
منابع مشابه
Identification of an essential Francisella tularensis subsp. tularensis virulence factor.
Francisella tularensis, the highly virulent etiologic agent of tularemia, is a low-dose intracellular pathogen that is able to escape from the phagosome and replicate in the cytosol. Although there has been progress in identifying loci involved in the pathogenicity of this organism, analysis of the genome sequence has revealed few obvious virulence factors. We previously reported isolation of a...
متن کاملIdentification of Francisella tularensis genes affected by iron limitation.
Cells of an attenuated live vaccine strain (LVS) of F. tularensis grown under iron-restricted conditions were found to contain increased quantities of several proteins relative to cells of this same strain grown under iron-replete conditions. Mass spectrometric analysis identified two of these proteins as IglC and PdpB, both of which are encoded by genes located in a previously identified patho...
متن کاملDiscrimination between Francisella tularensis and Francisella-like endosymbionts when screening ticks by PCR.
The presence of Francisella-like endosymbionts in tick species known to transmit tularemia poses a potential diagnostic problem for laboratories that screen tick samples by PCR for Francisella tularensis. Tick samples initially considered positive for F. tularensis based on standard 16S rRNA gene PCR were found to be positive only for Francisella-like endosymbionts using a multitarget F. tulare...
متن کاملAllelic exchange in Francisella tularensis using PCR products.
We describe here a technique for allelic exchange in Francisella tularensis subsp. novicida utilizing polymerase chain reaction (PCR) products. Linear PCR fragments containing gene deletions with an erythromycin resistance cassette insertion were transformed into F. tularensis. The subsequent ErmR progeny were found to have undergone allelic exchange at the correct location in the genome; the m...
متن کاملRhombencephalitis caused by Francisella tularensis.
Common presentations of tularemia include pneumonia and ulceroglandular, oropharyngeal, or typhoidal disease. Neuromeningeal involvement is extremely rare. We report a case of a severe rhombencephalitis due to Francisella tularensis. Diagnosis was possible thanks to a very precise interview, and the patient dramatically improved after specific antibiotherapy.
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ژورنال
عنوان ژورنال: FEMS Microbiology Ecology
سال: 1995
ISSN: 0168-6496
DOI: 10.1016/0168-6496(94)00072-5